Nitrofuran(AHD) rapid test strips (Egg)

Catalog No. LSY-20052H1d

1. Brief

This product is used for testing Nitrofuran(AHD) in eggs qualitatively. The whole test only needs 30-45min, easy to operate with high sensitivity.

 

2.Specifications    96 tests/kit

 

3. Components

1	Nitrofuran(AHD) test strips and micro-wells reagent	8pieces/bottle, 12 bottles
2	Manual	1 piece
3	Reagent A (100ml/bottle)	2 bottles
4	Reagent B (60ml/bottle)	1 bottle
5	Reagent C (100ml/bottle)	7 bottles
6	Reagent E (60ml/bottle)	2 bottles
7	Reagent F (100ml/bottle)	2 bottles

 

4. Application

Eggs, duck eggs etc.

 

5. Detection limit: 0.5μg/kg(ppb)

 

6. Date of manufacture and batch number: See bag and outer box for details.

 

7. Principle

The colloidal gold immuno-chromatography was used to detect the Nitrofuran(AHD) residue in the samples. The binding of Nitrofuran(AHD) to the specific monoclonal antibody labeled with colloidal gold inhibited the binding of the antibody to the conjugates of Nitrofuran(AHD)-BSA on the NC membrane Testing line, which resulted in the color depth variation of the testing line.

 

8. Testing equipment and materials required

Instruments: balance, centrifuge, sample concentrator, micropipette, oscillator (optional), homogenizer

Consumables: 10ml centrifuge tube, 50ml centrifuge tube, disposable tips, ethyl acetate.

 

9. Sample preparation

1)Take 5±0.05g of homogenized egg liquid sample into 50ml centrifuge tube, add 4.5ml deionized water, 1.5ml Reagent A and 300ul Reagent B, shake for 2 times, put at 75℃ water bath for 5min;

2) Add 7ml Reagent C, shake for 1min, then add 6ml ethyl acetate, shake for 1min, centrifuge at 4000r/min for 5min;

3) Take 2ml up-layer liquid into a 10ml centrifuge tube, blow to dry at 75℃ by Nitrogen or air.

4) Add 1ml Reagent E, shake to dissolve the dry residue (if do not dissolve the residue completely, it may lead to the result unstable), then add 1.2ml Reagent F, shake to even for 30s, be stable until layered(if appear emulsion, centrifuge at 4000r/min for 5min), discard the up-layer liquid, take down-layer liquid for testing.

Note: The treated sample can be used for testing for Nitrofuran(AMOZ, AOZ,AHD, SEM)

 

10. Operation procedures

10.1 Read the instruction carefully before use. Return test strips and sample into room temperature.

10.2 Take bottles needed from the kit package, take out required microwells and strips, making proper marks. Please use these test strips within 1h. Seal the cap of the bottles, avoid moisture.

10.3 Take 200ul of the test samples into the microwells, then slowly aspirate for many times, mix thoroughly with the freeze-dried powder in the micro-wells (this step is important).

10.4 After incubating for 5min at room temperature (20-40℃), then insert the test strip into the micro-well (with the MAX line end facing downwards, allowing it to be fully immersed in the solution).

10.5 After immersing the test strip in the micro-well for 5 minutes, take out the strip and interpret the results according to the schematic diagram. Please interpret the results within 1 minutes, otherwise interpretation will be invalid.

 

11. Test Result Interpretation

11.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Nitrofuran(AHD) residue in sample or the residue is lower than detection limit.

11.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Nitrofuran(AHD) residue is equal to or higher than detection limit.

11.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.   

 

 

12. Precautions

1) The test strip can be used only once at room temperature, do not use test strips out of expiry date.

2) Please seal the tube after taking out required test strips. If can not use all 8 micro-wells at once, cup the rest and put it back to tube and sealed.

3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.

4) Egg samples should be fully liquid without any agglomeration and deposition.

5) Use the test strips testing again for positive results.

6) The results is for reference only, for confirmation, please refer to the relevant national standard methods.

7) Please contact the supplier for any questions.

 

13. Safety performance and garbage disposal

This product's test strips and packaging are non-toxic and harmless, and should be disposed of as dry waste; The organic reagents used are collected and centrally processed in waste liquid bottles.

 

14. Storage and expiry date

Storage:  Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months.

 

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788  

Fax 86-755-28938800

Email: info@lsybt.com   

Website: www.lsybt.com