Sulfonamides residues (SAs) rapid test strip (Egg)
Catalog No. LSY-20040
1. Specifications: 8 strips/bottle, 12 bottles/kit
2. Brief
This product is used for testingSulfonamides residue in egg and duck egg sample. The total test time only need 10 min, fast with high sensitivity.
3. Detection limit ppb(ug/kg)
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Sulfadiazine (SD or SDZ)
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10-15
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Sulfamethoxazine
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50-60
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Sulfamethazine
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10-15
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Sulfachloropyridazine
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40-50
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Sulfamethoxine
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8-10
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Sulfapyridine
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40-50
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Sulfadimethoxine
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10-15
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Sulfadoxine
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40-50
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Sulfathiazole
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15-20
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Acesulfame
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50-60
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Sulfamethoxazine
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15-20
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Sulfapyrazole
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50-60
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Sulfaquinoxaline
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40-50
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Sulfadiazine
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20-30
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Sulfamethoxazole
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15-20
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Sulfaoxazole
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40-50
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Sulfadimethylpyrimidine
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15-20
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Sulfapyridine
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20-30
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Sulfaisoxazole
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50-60
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Sulfaphenazole
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10-15
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4. Principle
TheSulfonamides rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Sulfonamides in the sample combined with Sulfonamides specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Sulfonamides-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the concentration of Sulfonamides residue in sample is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color, the result is Positive.When the sample has no Sulfonamides residue or concentration lower than detection limit, T line is darker than C line, the result is Negative; No matter whether there is Sulfonamides residue in sample, C line will appear, it means the test is valid.
5. Contents
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1
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Sulfonamides test strips
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8 strips/bottle, 12 bottles
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2
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Red Micro-well
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8 wells/bottle, 12 bottles
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3
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Manual
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1 piece
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4
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Desiccant
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2 pieces/bag
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6. Sample preparation
Egg
Take 1g fresh homogeneous egg sample, add 3ml pure water, shake by hand or vortex strongly for 3min, ready for testing.
7.Operation procedures
7.1 Return sealed test strips, micro-wells and sample into room temperature.
7.2 Take necessary strips and micro-wells on desk, use it in 1 hour.
7.3 Take 150ul of the test samples into the micro-wells, then repeatedly absorb for 5-6 times, mix the sample with the reagent in the micro-wells completely.
7.4 Incubate for 3min at room temperature (20-25℃), then insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.
7.5 React at room temperature (20-25℃) for 3min, take out the test strips and read the result.
8. Test Result Interpretation
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Color depth comparison of T line and C line
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Result judgment
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Result analysis
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T line > C line
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Negative
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Sulfonamides residue is lower than detection limit in sample
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T line = C line
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Weak positive
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Sulfonamides residue is equal to detection limit in sample
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T line < C line or T line is invisible
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Positive
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Sulfonamides residue is higher than detection limit in sample
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C line is invisible
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Invalid
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The test dipstick is invalid, test again
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9.Specificity
Test 500ppb Chloramphenicol, quinolones and tetracyclines and other drugs. All the results are negative.
10.Precautions
1) The test dipsticks can be used only once at room temperature, do not use test dipsticks out of expiry date.
2) Please seal the bottle after taking out required test strips. If can not use all 8 micro-wells at once, cup the rest and put it back to bottle and sealed.
3) Do not touch the white membrane surface in the middle of test dipsticks, avoid sunlight and fan blowing directly.
4) Egg samples must be homogeneous and free of agglomeration, acidification and precipitation.
5) Use the test dipsticks testing again for positive results.
6) Please contact the supplier for any questions.
7) The result is for reference only, for confirmation, please refer to the relevant national standard method.
11.Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.