Brucella Antigen rapid test kit
Catalog No. LSY-20076-2
1.
Principle
The Brucella antigen rapid
test card uses colloidal gold immunochromatography technology, which is
suitable for qualitative detection of brucella. The brucella antigen in the
sample binds to the colloidal gold-labeled antibody to form a complex. During
the flow to the other end of the NC membrane, the complex binds to the brucella
antibody coated in the T-zone, and the T-line emerges. Line C is the control
line. The presence of line C indicates that the test strip is effective.
2. Specifications: 40 tests/kit
3. Components
|
1
|
Brucella antigen rapid test card
|
40 pieces
|
|
2
|
Cotton Swab
|
40 pieces
|
|
3
|
Dropper
|
40 pieces
|
|
4
|
Sample buffer
|
40tubes
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|
5
|
Manual
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1 piece
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4.Sample preparation
1). Genital secretions:
Take the secretions with a cotton swab and squeeze them repeatedly in the
sample buffer to fully dissolve and mix;
2). Abortion tissue, etc. :
Take about 0.5g tissue sample, add sample buffer, grind, mix well, stand for
10min or 5000r centrifuge for 3min;
3). Samples of blood,
saliva or milk: separated and prepared by conventional methods. Use dropper to
add 1 drop of sample buffer and mix it evenly.
5.Operation procedures
1) Read the manual carefully and return the test
card and sample to room temperature before use.
2) Take out test card, use dropper to absorb the
prepared sample, drop 2-3 drops of sample into well mark “S”;
6.Results
Negative(-): no color reaction on
Test Line (T Line), only on Control Line(C Line) being seen red color reaction.
It means there is no Brucella Ag in sample or the levels is lower than the
detection threshold;
Positive(+): Both on T Line and C line being seen red color reaction. It means there
is Brucella Ag in sample;
Invalid:No color reaction on C Line. It means the
operation is wrong or the test card is invalid.
7. Notice
1). The
test card can be used only once at room temperature, do not reuse or use test
card out of expiry date.
2).Return
all reagents and sample into room temperature before use, after the test card
is unsealed, please use it as soon as possible.
3). This
product is only for rapid in vitro diagnosis by veterinarians and cannot be quantified.
It is only for qualitative testing.
4). The
test results of this product are for reference only. If confirmation is
required, please refer to relevant national standards and methods.
5).
During acute infection with Brucella, pathogenic microorganisms are easily
detected, especially in samples related to the reproductive system; In chronic
infections, it is easy to detect during the detoxification period, but not during
the non detoxification period. At this time, serum antibodies are often tested as
reference indicators.
6). It is
important to pay attention to biosafety protection during testing, and the
waste after testing should be disposed of for relevant biosafety.
7). The
positive result of unvaccinated vaccine can be preliminarily determined as wild
virus infection; After vaccination, due to the different duration of existence
of different vaccines in the body, they generally disappear after 50-180 days
of immunization. Positive results beyond this time can be preliminarily
determined as wild virus infection. (Brucella S2 strain has a maximum survival
time of 50 days; A19 has a maximum survival time of 90 days; M5 has a maximum
survival time of 180 days)
8). This
product can be used for regular screening and testing, and for positive
results, more specific methods can be used for confirmation.
8.Storage
and expiry date
Storage: Store at
4-30 ℃ in dark, sealed, dry place, no frozen.
Expiry
date:24 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building,
National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,
Shenzhen, 518120 China
Tel.
86-755-28438788
Fax
86-755-28938800
Email: info@lsybt.com
www.lsybt.com