Malachite green rapid test card (Water:2ppb)

Catalog No. LSY-20005-1W

1. Specifications

20 pieces/kit

2. Brief

This product is used for testing Malachite green or Leucomalachite Green in water sample (including pond water, etc.) qualitatively. Easy to operate, high sensitivity.

3. Detection limit

Water:  2ng/ml（ppb）

4. Contents

5. Principle

The Malachite green rapid test card is based on competitive inhibition immuno-chromatographic principle. In the flow process, Malachite green in the sample combined with Malachite green specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Malachite green-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Malachite green residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Malachite green residue in sample, C line will appear, it means the test is valid.

6. Materials required but not provided

1) Equipment: Centrifuge, Micropipette, balance (a reciprocal sensibility of 0.01 g);

2) Consumables: 15ml centrifuge tube, disposable tips.

7. Sample preparation

Water sample:

No need for treatment (if there are too many impurities, centrifuge at 4000 rpm for 5 minutes, or let it stand), directly take 1.8ml of water sample and add 0.2ml of reagent 1, mix well and wait for testing.

8. Operation procedures

8.1 Read the instruction carefully before use. Return test cards and sample into room temperature.

8.2 Take out test bag, open and take out required micro-wells, making proper marks. Please use them within 1h. Seal the test bag, avoid moisture.

8.3 Take 120µl of the test samples into the micro-wells, then repeatedly pump and suck for many times, mix the sample with the reagent in the micro-wells completely until no solid judged by eyes (this is a very important step). Incubate for 2min at room temperature (20-25℃).

8.4 Put the test card flatly, absorb the 80µl liquid from the micro-well, add into the “S” hole on test card.

8.5 Be static, read the result in 5~8min, it is invalid in other time.

9. Test Result Interpretation

9.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Malachite green residue in sample or the residue is lower than detection limit.

9.2 Positive: T line is obviously lighter than C line or T line is invisible or T line appear green or blue color. It means the Malachite green residue is equal to or higher than detection limit.

10. Precautions

1) The test card can be used only once at room temperature, do not use test card out of expiry date.

2) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.

3) Test again for positive result;

4) Please contact the supplier for any questions.

11.Specificity

Test 500ppb Tetracyclines, Quinolones and Sulfonamides etc. The result is Negative.

12. Storage and expiry date

Storage:  Store at 4-30 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788 

Fax 86-755-28938800

Email: info@lsybt.com   

Website: www.lsybt.com