Crystal violet rapid test card (Tissue 0.5ppb)

Catalog No.: LSY-20115B

1. Specifications

10 pieces/kit

2. Brief

This product is used for testing Crystal violet and Leuco crystal violet in Tissue (include Fish, Shrimp etc.) sample qualitatively. Easy to operate, high sensitivity.

3. Detection limit

Tissue:  0.5ng/g (0.5ppb)

4. Contents

1	Crystal violet rapid test card (include test card, desiccant, micro-wells reagent)	10 T
2	Manual	1 piece
3	Reagent 1	1 bottle
4	Reagent 2	2 bottles
5	Reagent 3	10 bottles
6	Reagent 4	1 bottle
7	Reagent 5	1 bottle

5. Principle

The Crystal violet rapid test card is based on competitive inhibition immuno-chromatographic principle. In the flow process, Crystal violet in the sample combined with Crystal violet specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Crystal violet-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Crystal violet residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Crystal violet residue in sample, C line will appear, it means the test is valid.

6. Materials required but not provided

1) Equipment: Homogenizer, nitrogen-drying device, vortex(optional), centrifuge, Micropipette, balance (a reciprocal sensibility of 0.01 g);

2) Consumables: 15ml centrifuge tube, disposable tips.

7. Sample preparation

1) Take 3±0.05g homogenized tissue sample into 15ml centrifuge tube, add 2ml Reagent 1, then add 6ml reagent 2, shake by hand or by vortex strongly for 2min (shake by hand up-and-down strongly), add 1 bottle of reagent 3, shake strongly for 2min (shake by hand up-and-down strongly), then centrifuge at 4000r/min for 5min;

2) Transfer 3ml up-layer clear liquid into another centrifuge tube, add 100μl reagent 4, shake for 30S. Blow to dry at 56℃ by Nitrogen or air(or use hair dryer);

3) Add 0.5ml reagent 5, shake for 2min (add a 200ul tips into the centrifuge tube) to fully dissolve the residue on wall of centrifuge tube, absorb 120ul down-layer liquid as sample, ready to test.

8. Operation procedures

8.1 Read the instruction carefully before use. Return test cards and sample into room temperature.

8.2 Take out test bag, open and take out required microwells, making proper marks. Please use them within 1h. Seal the test bag, avoid moisture.

8.3 Take 120ul of the test samples into the microwells, then repeatedly pump and suck for many times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step). Incubate for 1-2min at room temperature (20-25℃).

8.4 Put the test card flatly, absorb the 80µl liquid from the micro-well, add into the “S” hole on test card.

8.5 Be static, read the result in 5~8min, it is invalid in other time.

9. Test Result Interpretation

9.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Crystal violet residue in sample or the residue is lower than detection limit.

9.2 Positive: T line is obviously lighter than C line or T line is invisible or T line appear green or blue color. It means the Crystal violet residue is equal to or higher than detection limit.

10. Precautions

1) The test card can be used only once at room temperature, do not use test card out of expiry date.

2) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.

3) Test again for positive result;

4) Please contact the supplier for any questions.

11.Specificity

Test 500ppb Tetracyclines, Quinolones and Sulfonamides etc. The result is Negative.

12. Storage and expiry date

Storage:  Store at 4-30 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788 

Fax 86-755-28938800

Email: info@lsybt.com   

Website: www.lsybt.com