Trimethoprim rapid test strip (Tissue)
Catalog No. LSY-20114
1. Specifications: 8 strips/bottle, 12 bottles/kit
2. Brief
This product is used for testing Trimethoprim in tissue(fish, shrimp,
crab, chicken, livestock etc.) sample. Easy to operate with high sensitivity.
3. Detection limit: 5ng/g (ppb)
4. Principle
The Trimethoprim rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Trimethoprim in
the sample combined with Trimethoprim
specific colloidal gold-labeled monoclonal antibody, Inhibit the combination
between antibody and Trimethoprim-BSA conjugate on Test line of NC membrane, lead
to the color change of Test line. When the concentration of Trimethoprim residue in sample is equal to or higher than
detection limit, T line is obviously lighter than C line or T line has no color, the result is Positive.When the sample has no Trimethoprim residue or concentration
lower than detection limit, T line is darker than C line, the result is Negative; No matter whether there is Trimethoprim residue in sample, C line
will appear, it means the test is valid.
5. Contents
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1
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Trimethoprim test strips
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8 strips/bottle, 12 bottles
|
|
2
|
Red Micro-well
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8 wells/bottle, 12 bottles
|
|
3
|
Manual
|
1 piece
|
|
4
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Desiccant
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2 pieces/bag
|
|
5
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Sample redissolving solution 10X
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1 bottle
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6. Solution preparation
Sample redissolving solution: Dilute Sample redissolving solution 10X with deionized water at 1:9: 1 part Sample redissolving
solution 10X + 9 parts deionized water.
7. Sample preparation
Take 3±0.05g homogenized tissue sample, add 3ml Sample redissolving solution, shake for 2min; centrifuge at 4000rpm
for 5min, take up-layer
clear liquid to test.
8. Operation
procedures
8.1 Return test strips and sample into room temperature before use.
8.2 Take necessary strips and micro-wells on desk, use it in 1 hour.
Seal the unused strips and microwells, avoid moisture.
8.3 Take 200ul of the test samples into the micro-wells, then slowly
aspirate multiple times to mix the sample with the reagent in the micro-wells
completely (this is a very important step).
8.4 Incubate for 5min at room temperature (20-25℃), then insert the test
dipsticks into the micro-wells with the "MAX" end fully dipped in to
the mixture solution.
8.5 React at room temperature (20-25℃) for 5min
again, take out the test strips and read
the result.
9. Test Result
Interpretation
9.1 Negative: T line is darker than C line, or T line has same color
with C line. It means there is no Trimethoprim residue in sample or the residue
is lower than detection limit.
9.2 Positive: T line is obviously lighter than C line or T line is
invisible. It means the Trimethoprim residue is equal to or higher than
detection limit.
10.Specificity
Test 500ppb Tetracyclines, Quinolones, Sulfonamides
and other drugs. All the results are negative.
11. Precautions
1) The test strips can be used only once at room
temperature, do not use strips out of expiry date.
2) Do not touch the white membrane surface in the middle of test strip,
avoid sunlight and fan blowing directly.
3) Use the test dipsticks testing again for
positive results.
4) Please contact the supplier for any
questions.
12. Storage and expiry date
Storage:
Store at 2-8
℃ in dark, sealed, dry place, no frozen.
Expiry date: 12
months; date of production is on box.
Shenzhen Lvshiyuan
Biotechnology Co., Ltd
D Building, National Biological Industrial Park
of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com