Catalog No.: CK27
INTENDED USE
The Leishmania canis Antibody Rapid Test isatestcassette to diagnose the presence ofLeishmania canisantibody(LSH Ab) indog’s blood specimen.
Assay Time: 5-10 minutes
Specimen: Serum, plasma
PRINCIPLE
The Leishmania canis Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay.
REAGENTS AND MATERIALS
- Test devices
- Disposabledroppers
- Assay buffers
- Products Manual
STORAGE AND STABILITY
The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.
SPECIMEN PREPARATION AND STORAGE
- Specimen should be obtained and treated as below.
- Serum or plasma: collect the whole blood for the patient dog, centrifuge it to get the serum, or place the whole blood into a tube which contains anticoagulants to get plasma.
- Whole blood: collect fresh blood for use directly or make anticoagulant blood for storage at 2-8℃.
- All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8℃.
TEST PROCEDURE
- Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.
- Take out thetest device from the foil pouch and place it horizontally.
- Collect 20μL of the prepared specimen into a vial of assay buffer and mix well. Then drop 3 drops (approx. 120μL) of the diluted sample into the sample hole “S” of the testcard, respectfully matching the windows. Start the timer.
- Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
INTERPRETATION OF RESULTS
- Positive (+): The presence of both “C”line and zone “T”line, no matter Tline is clear or vague.
- Negative (-): Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.
PRECAUTIONS
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not use the test beyond its expiration date.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
- All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
LIMITATION
The Leishmania canis Antibody Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian.It is suggested to apply a further confirmative method such asPCR or microscopywhen positive result was observed.