Product For Ruminants

LSY-20091 Rotavirus antigen rapid test kit

Rotavirus antigen rapid test kit (Colloidal gold method)

Catalog No. LSY-20091

1. Introduction

The Rotavirus antigen rapid test kit is based on colloidal gold immuno-chromatography technique to detect type A Rotavirus antigen in feces of cattle, pig, dog and cat. After adding sample, the sample move along with the colloidal gold labeling Rotavirus antibody, if there is Rotavirus antigen in the sample, it combines with antibody on T line and show wine-red color; if there is no Rotavirus antigen, then no color reaction.

2. Contents

1

RV Ag test card

40 pieces

2

Swab

40 pieces

3

Dropper

40 pieces

4

Sample buffer

40 bottles

5

Manual

1 piece

3. Test procedure

1) Use swab to collect fresh feces or take sample from Rectum directly. If it is for Rotavirus screening for animals without diarrhea, please take sample from Rectum. We suggest that do not take dry feces sample, because collecting dry feces sample will lead to collecting too much sample and appear false positive.

2) Insert the swab into the tube with sample buffer immediately, rotate the swab strongly on tube well for at least 10 times and mix the solution evenly, making the sample been dissolved in sample buffer fully. Press the swab on tube well above the liquid level, making the liquid been pressed out fully, discard swab.

3) If the feces particles is big, be static for 1-2min, make big particles precipitate.

4) Take out test card from sealed bag. Put it on flat surface, use dropper to draw upper-layer bright liquid, drop 5 drops (do not over adding) slowly, one by one, to the sample hole with “S” mark.

5) Put the test card at room temperature for 10-15min, read the result, the result is invalid after 15 minutes.

4. Results judgment


          

1) Positive: control line and test line are both seen wine red;

2) Negative: only control line is seen wine red;

3) Invalidation: control line isn’t seen wine red.


5. Note

1). Products that have expired or have damaged aluminum foil packaging bags cannot be used.

2). The test card must be used within 1 hour after removal.

3). Please use the accompanying sample buffer. Do not mix Sample buffer between different products or Sample buffer from different batches of the same product.

4). The collected sample should be tested immediately, avoid long time storage.

5). The collection of samples directly affects the accuracy of testing, so ensuring the correct, sufficient, and appropriate collection of samples. Excessive collection of fecal samples can affect the normal flow of fluids and lead to incorrect results (black bands appearing), while too few samples may result in false negative results. Therefore, please control the sampling amount according to the diagram

 


6). Avoiding bubbles when adding samples to the sample hole of the test card. When adding samples, please keep the front end of the straw about 1 cm away from the sample hole to ensure accurate adding samples; If the distance is too close, the drop volume may be reduced or the sample size may not be added due to the error in judging the number of drops, which will affect the test effect

7). Used test cards and samples should be considered as pollutants and must be properly disposed of in accordance with relevant local regulations

6. Frequently Asked Questions

1) Limitation of the product

This kit is a qualitative screening reagent, can detect RV antigen accurately, but if sample is not enough or concentration of antigen is lower than sensitivity, it may appear negative result. A clear clinical diagnosis should be made after taking into account all clinical and laboratory phenomenon.

2) The sensitivity and specificity of the product.

Compare with PCR, the sensitivity > 95%, it has no cross-reaction with similar symptoms to those of Coronavirus, adenovirus, Canine parvovirus, Escherichia coli, infectious gastroenteritis, epidemic diarrhea virus, bovine infectious diarrhea virus

3) Whether there will be Positive reaction after vaccination.

RV vaccination won’t make RV antigen appear in feces samples, so vaccination won’t make positive result.


7. Storage and expiry date

Specifications: 40T/kit

Storage: Store at 2-30 ℃ (No frozen). Return to room temperature before open the Aluminum foil bags for cold storage.

Expiry date: 24 months; date of production is on box.


Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788        

Fax 86-755-28938800

Email:   info@lsybt.com

www.lsybt.com

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Company: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

WeChat/WhatsApp: +86-13427908554

Mobile: +86-18165709090 Skype: bellazou3

E-mail: info@lsybt.com, lsy@lsybt.com

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