Sulfamethazine (SM2) ELISA Kit for meat safety
- 2026-09-16 佚名
Sulfamethazine (SM2) ELISA Kit
Catalog No. LSY-10010
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Sulfamethazine (SM2) residue. The coupling antigens are pre-coated on the micro-well stripes. The Sulfamethazine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfamethazine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfamethazine in the sample. This value is compared to the standard curve and the Sulfamethazine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Tissue (high-detection-limit method), feed 1 ppb
Tissue (lower-detection-limit method)……………………………………...5 ppb
Honey, egg 1 ppb
Serum, urine 4 ppb
Milk 20 ppb
Cross-reaction rate
Sulfamethazine(SM2)………………………………………………………. 100%
Recovery rate
Tissue, urine, milk, feed 85±25%
Honey, serum, egg 80±23%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 1ppb |
3ppb | 9ppb | ||
27ppb | 81ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 20×concentrated redissolving solution | 50ml | transparent cap |
4. Materials required but not provided
1) Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, votex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g), Incubator
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30-300 µL;
3) Reagents: Acetonitrile (CH3CN), ethyl acetate, N-hexane, K2HPO4·12H2O, Citric acid monohydrate (C6H8O7·H2O), HCl, NaOH, CH2Cl2,
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 1 year; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com