Sulfaquinoxaline ELISA Test Kit for honey
- 2026-09-16 佚名
Sulfaquinoxaline ELISA Test Kit
Catalog No. LSY-10015
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Sulfaquinoxaline in samples. The conjugated antigens are pre-coated on the micro-well stripes. The Sulfaquinoxaline in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Sulfaquinoxaline concentration in the sample. This value is compared to the standard curve and Sulfaquinoxaline concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Incubator temperature: 25℃
Incubator time: 60min～(20~30)min
Detection limit:
Tissue, honey 1 ppb
Serum, urine 4 ppb
Milk……………………………………………………………………………20 ppb
Cross-reaction rate
Sulfaquinoxaline (SQX) 100%
Recovery rate:
Tissue 90 ±22%
Milk, honey, serum 83 ±17%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 1 ppb, 3 ppb, 9 ppb, 27 ppb and 81 ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (10 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap
4. Materials required but not provided
1) Equipments: microplate reader, printer, mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, balance(a reciprocal sensibility of 0.01 g);
2) Micropipettors: single-channel 20-200 µL and 100-1000 µL, and multi-channel 30～300 µl;
3) Reagents: Ethyl acetate, N-hexane, Na2HPO4·12H2O, NaH2PO4·2H2O, NaOH, HCl
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Remarks: If the vacuum package of microtiter plates has leakage, the microtiter plate is normal and effective, do not affect the experimental result. Please feel free to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: bella.lsybt@gmail.com info@lsybt.com
www.lsybt.com