Sulfamethoxazole(SMZ) ELISA Kit for honey
- 2026-09-16 佚名
Sulfamethoxazole(SMZ) ELISA Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Sulfamethoxazole(SMZ) residue. The coupling antigens are pre-coated on the micro-well stripes. The Sulfamethoxazole(SMZ) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfamethoxazole(SMZ) antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfamethoxazole(SMZ) in the sample. This value is compared to the standard curve and the Sulfamethoxazole(SMZ) concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Tissue (high-detection-limit method) 1 ppb
Tissue (lower-detection-limit method)……………………………………...5 ppb
Honey 1 ppb
Serum, urine 4 ppb
Milk 20 ppb
Cross-reaction rate
Sulfamethoxazole (SMZ) ….. 100%
Sulfamerazine (SM1) 12%
Phthalylsulfathiazole (PST) 13%
Sulfapyridine 28%
Sulfamethoxydiazine(SMD) 26.4%
Recovery rate
Tissue, urine, milk 85±25%
Honey, serum 80±23%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 1ppb, 3ppb, 9ppb, 27ppb and 81ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 20× concentrated redissolving solution (50 mL) transparent cap
4. Materials required but not provided
1) Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, votex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g), Incubator
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30-300 µL;
3) Reagents: Acetonitrile (CH3CN), ethyl acetate, N-hexane, K2HPO4·12H2O, Citric acid monohydrate (C6H8O7·H2O), HCl, NaOH, CH2Cl2,
5. Storage and expiry date
Storage: store at 2-8℃, not frozen.
Expiry date: 12 months; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: bella.lsybt@gmail.com info@lsybt.com
www.lsybt.com