Cephalosporin ELISA Test Kit For Fish
- 2026-10-19 佚名
Cephalosporin ELISA Test Kit
Catalog No. LSY-10077
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Cephalosporin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Cephalosporin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cephalosporin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Cephalosporin in it. This value is compared to the standard curve and the Cephalosporin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Standard curve range: 1ppb-27ppb;
Intra-plate variation coefficient:<5%;
Variation coefficient between plates:<15%;
The best value of B0 absorbance:>0.8;
Incubation Temperature: 25℃
Incubation Time: 30min—30min—15min
Detection limit:
Tissue(method 1) about 30ppb
Tissue(method 2) about 4ppb
Milk about 10ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate
Ceftiofur 100%
DesfuroylCeftiofur 100%
Cefquinome 103%
Ceftiaxone 157%
Cefotaxime 60%
Recovery rate:
90%±30%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） Standard solution (1ml/bottle): 0ppb, 1ppb, 3ppb, 9ppb, 27ppb, 81ppb.
3） Antibody working solution (7 mL)
4） Enzyme labeled II antibody working solution (12 mL)
5） 20× concentrated washing buffer (30 mL)
6） 10× concentrated redissolving solution (10 mL)
7） Substrate A (7 mL)
8） Substrate B (7 mL)
9） Stop solution (7 mL)
4. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com