Ampicillin ELISA Test Kit for fish shrimp crab
- Sep 01, 2026 Anon
Ampicillin ELISA Test Kit
Catalog No. LSY-10018
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Ampicillin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Ampicillin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Ampicillin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ampicillin in it. This value is compared to the standard curve and the Ampicillin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Standard curve range: 0.05ppb-4.05ppb;
Intra-plate variation coefficient:<5%;
Variation coefficient between plates:<15%;
The best value of B0 absorbance:>0.8;
Incubation Temperature: 25℃
Incubation Time: 30min—10~15min
Detection limit:
Tissue, egg, pure milk about 1ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate
Ampicillin 100%
Recovery rate:
90%±30%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） Standard solution (1ml/bottle): 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb.
3） 11X Concentrated Enzyme conjugate (0.7 mL)
4） Enzyme conjugate dilution (7 mL)
5） 20× concentrated washing buffer (30 mL)
6） Sample diluent solution (20 mL)
7） Substrate A (7 mL)
8） Substrate B (7 mL)
9） Stop solution (7 mL)
4. Materials required but not provided
1) Equipment: microplate reader (450nm, 630nm), rotary evaporator/nitrogen-drying device, homogenizer, oscillator, centrifuge (4000g and above), balance (a sensibility reciprocal of 0.01 g), measuring pipets, incubator (adjustable 25℃),timer
2) Micropipette: single-channel 20~200 µL and 100~1000 µL, and eight-channel 30~300 µL;
3) Reagents: Deionized water, Acetonitrile (analytical reagent).
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
