Cefalexin ELISA Test Kit for fish shrimp
- Sep 01, 2026 Anon
Cefalexin ELISA Test Kit
Catalog No. LSY-10067
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Cefalexin in samples. The coupling antigens are pre-coated on the micro-well stripes. The Cefalexin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cefalexin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Cefalexin in the sample. This value is compared to the standard curve and the Cefalexin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.3 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue 10ppb
Egg 15ppb
Liquid milk 5ppb
Milk powder 24ppb
Cross-reaction rate:
Cefalexin 100%
Recovery rate
Tissue 100%±30%
Egg, liquid milk, milk powder 90%±30%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.3ppb |
0.9ppb | 2.7ppb | ||
8.1ppb | 24.3ppb | ||
3 | High concentration standard | 1ml | 1 ppm |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A solution | 7ml | white cap |
7 | Substrate B solution | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× extracting buffer A | 15ml | yellow cap |
10 | 2× extracting buffer B | 50ml*2 | transparent cap |
11 | 20× concentrated washing buffer | 15ml | white cap |
12 | 10× concentrated redissolving solution | 10ml | black cap |
4. Materials required but not provided
1) Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g), incubator.
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 µl ;
3) Reagents: Methanol, NaOH
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 1 year; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
