Amoxicillin ELISA Test Kit for seafood
- Sep 01, 2026 Anon
Amoxicillin ELISA Test Kit
Catalog No. LSY-10054
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Amoxicillin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Amoxicillin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Amoxicillin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Amoxicillin in it. This value is compared to the standard curve and the Amoxicillin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue, Egg, Milk 4ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate:
Amoxicillin 100%
Recovery rate:
80%±15%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） Standard solution (1ml/bottle): 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb.
3） 11X Concentrated Enzyme conjugate (0.7 mL)
4） Enzyme conjugate dilution (7 mL)
5） Substrate A (7 mL)
6） Substrate B (7 mL)
7） Stop solution (7 mL)
8） 20× concentrated washing buffer (30 mL)
9） Sample diluent (20 mL)
4. Materials required but not provided
1) Equipments: microplate reader (450nm, 630nm), rotary evaporator/nitrogen-drying device, homogenizer, oscillator, centrifuge (3000g and above), balance (a sensibility reciprocal of 0.01 g), measuring pipets, incubator (adjustable 25℃),timer
2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and eight-channel 30~300 µL;
3) Reagents: Deionized water, Concentrated hydrochloric acid (analytical pure)
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com www.lsybt.com
