Tylosin ELISA Test Kit for fish shrimp
- Sep 01, 2026 Anon
Tylosin ELISA Test Kit
Catalog No. LSY-10020
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Tylosin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tylosin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. This value is compared to the standard curve and the Tylosin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Tissue(method 1) 6 ppb
Tissue(method 2) 7.5 ppb
Cross-reaction rate
Tylosin 100%
Recovery rate
Tissue 70%~120%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.5ppb |
1.5ppb | 4.5ppb | ||
13.5ppb | 40.5ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 15ml | white cap |
9 | 20× concentrated extraction A | 15ml | yellow cap |
10 | 2× concentrated extraction B | 50ml*2 | transparent cap |
11 | 10× concentrated sample dilution | 10ml | black cap |
4. Materials required but not provided
1) Equipments: microplate reader (450 nm / 630 nm), homogenizer, oscillator, votex, measuring pipets, centrifuge, and balance (a sensibility reciprocal of 0.01 g), incubator, printer.
2) Micropipettors: single-channel 20~200µL and 100~1000µL, and multi-channel 30～300 µl;
3) Reagents: NaOH, deionized water.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Remarks: If the vacuum package of microtiter plates has leakage, the microtiter plate is normal and effective, do not affect the experimental result. Please feel free to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
