Tylosin ELISA Test Kit for honey
- Sep 01, 2026 Anon
Tylosin ELISA Test Kit (for honey)
Catalog No. LSY-10020H
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tylosin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tylosin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. This value is compared to the standard curve and the Tylosin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Standard curve range: 0.2 ppb - 16.2 ppb;
Coefficient of variation within the plates: <5%;
Coefficient of variation between plates: <15%;
Best value of B0 absorbance: >0.8
Detection limit
Honey about 0.5ppb
Note: ppb= ng/ml or ng/g
Cross-reaction rate
Tylosin 100%
Recovery rate
90±30%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb
3） 11X Concentrated Enzyme conjugate (0.7 mL)
4） Enzyme conjugate dilution (7 mL)
5） Substrate A (7 mL)
6） Substrate B (7 mL)
7） Stop solution (7 mL)
8） 20× concentrated washing buffer (30 mL)
9） 5× concentrated redissolving solution (10 mL)
4. Materials required but not provided
1） Equipment: microplate reader (450nm, 630nm), printer, homogenizer, nitrogen-drying device, vortex, shaker, centrifuge (4000g and above), measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator (25℃), water bath, timer;
2） Micropipette: single-channel 20-200 µL, 100-1000 µL, and eight-channel 30～300 µl;
3） Reagents (AR): NaOH, ethyl acetate, deionized water.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
