Gentamicin ELISA Test Kit for fish shrimp crab
- Sep 01, 2026 Anon
Gentamicin ELISA Test Kit
Catalog No. LSY-10023
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Gentamicin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Gentamicin in the sample and pre-coated coupling antigen on the micro-well stripes compete for the anti-Gentamicin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Gentamicin in it. The value is compared to the standard curve and the Gentamicin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Tissue 2.5 ppb
Liver, milk 3 ppb
Recovery rate
Tissue 90±20%
Liver 80±20%
Milk 90±18%
Cross-reaction rate
Gentamicin 100%
Streptomycin <1%
Dihydrostreptomycin <1%
Neomycin <1%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.05ppb |
0.15ppb | 0.45ppb | ||
1.35ppb | 4.05ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 15ml | white cap |
9 | Sample redissolving solution | 50ml | transparent cap |
10 | 10× concentrated extracting solution | 50ml | blue cap |
4. Materials required but not provided
1) Equipments: microplate reader, votex, centrifuge, homogenizer, measuring pipets, balance (a sensibility reciprocal of 0.01 g).
2) Micropipettors: single-channel 20-200 µL and 100-1000 µL, and multi-channel 30～300 µL;
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
