Streptomycin ELISA Test Kit for milk
- Sep 01, 2026 Anon
Streptomycin ELISA Test Kit
Catalog No. LSY-10024
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Streptomycin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Streptomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti- Streptomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Streptomycin concentration in the sample. This value is compared to the standard curve and the Streptomycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue/Aquatic product (Method 1), honey, milk 4 ppb
Tissue/Aquatic product (Method 2) 2 ppb
Milk powder 20 ppb
Recovery rate:
Tissue/Aquatic product 70%～120%
Honey, milk, milk powder 80%～120%
Cross-reaction rate:
Streptomycin 100%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.2ppb |
0.6ppb | 1.8ppb | ||
5.4ppb | 16.2ppb | ||
3 | Spiking standard solution | 1ml | 1ppm |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A solution | 7ml | white cap |
7 | Substrate B solution | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20X Sample extraction A | 15ml | yellow cap |
10 | 2X Sample extraction B | 50ml*2 | transparent cap |
11 | 20X concentrated washing buffer | 15ml | white cap |
5X Sample extraction C | 10ml | black cap | |
13 | 20X Sample dilution | 15ml | transparent cap |
4. Materials required but not provided
1) Equipments: microplate reader, printer, homogeniser, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance( a reciprocal sensibility of 0.01 g).
2) Micropipettors: single-channel 20~200 µL, 100~1000 µL; and multi-channel 30～300 μl;
3) Reagents: Methanol, HCl, NaOH, Trichloroacetic acid
5. Storage and expiry date
Storage: stored at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box
Note: If the vacuum package of microplate has leakage, it is still valid, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
