Malachite Green ELISA Test Kit for seafood
- Sep 01, 2026 Anon
Malachite Green ELISA Test Kit
Catalog No.: LSY-10027
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Malachite Green in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Malachite Green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Malachite Green antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite Green in it. This value is compared to the standard curve and the Malachite Green concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.03 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue about 0.2ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate:
Malachite Green 100%
Leucomalachite green(after sample preparation) 100%
Crystal Violet 100%
Leucocrystal Violet(after sample preparation) 100%
Recovery rate:
85%±20%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) Standard solution: 0 ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb,2.43ppb (1ml/bottle)
3) 11X Concentrated Enzyme conjugate (0.7 mL)
4) Enzyme conjugate dilution (7 mL)
5) 20× concentrated washing buffer (30 mL)
6) Redissolving solution (100 mL)
7) Sample extractant 1 (10 mL)
8) Sample extractant 2 (6 mL)
9) Substrate A (7 mL)
10) Substrate B (7 mL)
11) Stop solution (7 mL)
4. Materials required but not provided
1) Equipments: microplate reader (450nm, 630nm), rotary evaporator/nitrogen-drying device, homogenizer, oscillator, centrifuge (4000g and above), balance (a sensibility reciprocal of 0.01 g), measuring pipets, incubator (adjustable 25℃),timer
2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and multi-channel 30~300 µL;
3) Reagents: Acetonitrile, Dichloromethane, N-hexane (or n-heptane),deionized water.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
