Oxytetracyline ELISA Test Kit for seafood
- Sep 01, 2026 Anon
Oxytetracyline ELISA Test Kit
Catalog No. : LSY-10044B
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Oxytetracyline in the fish and shrimp sample. The coupling antigens are pre-coated on the micro-well stripes. The Oxytetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Oxytetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Oxytetracyline in it. This value is compared to the standard curve and the Oxytetracyline concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.3 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Fish, shrimp 1.5ppb
Cross-reaction rate:
Oxytetracyline 100%
Tetracycline 200%
Doxycycline 8.5%
Chlortetracycline 21%
Recovery rate:
Fish, shrimp 60%～120%
Precision
Within batch coefficient of variation ≤ 15%, between batch coefficient of variation ≤ 15%
Applicable scope
Can qualitatively and quantitatively detect the Oxytetracyline residual amount in fish and shrimp sample.
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.3ppb |
0.9ppb | 2.7ppb | ||
8.1ppb | 24.3ppb | ||
3 | Spiking standard solution | 1ml | 1 ppm |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× concentrated washing buffer | 15ml | white cap |
10 | 2× sample extract | 100ml*2 | transparent cap |
4. Materials required but not provided
1) Equipment: microplate reader, printer, homogenizer, oscillator, centrifuge, balance (a sensibility reciprocal of 0.01 g), graduated pipette.
2) Micropipette: single-channel 20~200 µL and 100~1000 µL, and multi-channel 250 µL;
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
