Oxytetracyline ELISA Test Kit for milk safety
- Sep 01, 2026 Anon
Oxytetracyline ELISA Test Kit
Catalog No. LSY-10044
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Oxytetracyline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Oxytetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Oxytetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Oxytetracyline in it. This value is compared to the standard curve and the Oxytetracyline concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.4 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue, Honey about 20ppb
Milk (method 1) about 20ppb
Milk (method 2) about 40ppb
Feed about 600ppb
Note: ppb=ng/mLor ng/g
Cross-reaction rate:
Oxytetracyline 100%
Tetracycline 200%
Chlortetracycline 240%
Doxycycline 20%
Recovery rate: 90±15%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 10× concentrated standard solution: 0 ppb, 4ppb, 12ppb, 36ppb, 108ppb (0.5ml/bottle)
3） Enzyme conjugate (7 mL)
4） Antibody working solution (7 mL)
5） Substrate A (7 mL)
6） Substrate B (7 mL)
7） Stop solution (7 mL)
8） 20× concentrated washing buffer (30 mL)
9） 20× concentrated redissolving solution (10 mL)
4. Materials required but not provided
1) Equipments: microplate reader (450nm, 630nm), rotary evaporator/nitrogen-drying device, homogenizer, oscillator, centrifuge (4000g and above), balance (a sensibility reciprocal of 0.01 g), measuring pipets, incubator (adjustable 25℃、37℃、60℃),timer
2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and multi-channel 30~300 µL;
3) Reagents: deionized water, HCl, N,N-Dimethylformamide(DMF)
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
