Chlortetracyline ELISA Test Kit for milk safety
- Sep 01, 2026 Anon
Chlortetracyline ELISA Test Kit
Catalog No. LSY-10049m
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chlortetracyline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Chlortetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Chlortetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chlortetracyline in it. This value is compared to the standard curve and the Chlortetracyline concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue about 3ppb
Honey about 5ppb
Milk (method 1) about 5ppb
Milk (method 1) about 10ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate:
Chlortetracyline 100%
Tetracyclines 80%
Oxytetracyline 40%
Doxycycline 10%
Recovery rate:
Tissue 90±15%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 10× concentrated standard solution (0.5ml/bottle): 0ppb, 2ppb, 6ppb, 18ppb, 54ppb
3） Enzyme conjugate (7 mL)
4） Antibody working solution (7 mL)
5） Substrate A (7 mL)
6） Substrate B (7 mL)
7） Stop solution (7 mL)
8） 20× concentrated washing buffer (30 mL)
9） 20× concentrated redissolving solution (10 mL)
4. Materials required but not provided
1) Equipments: microplate reader (450nm, 630nm), rotary evaporator/nitrogen-drying device, homogenizer, oscillator, centrifuge (4000g and above), balance (a sensibility reciprocal of 0.01 g), measuring pipets, incubator (adjustable 25℃、37℃、60℃),timer
2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and multi-channel 30~300 µL;
3) Reagents: deionized water
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
