Doxycycline ELISA Test Kit for seafood safety
- Sep 01, 2026 Anon
Doxycycline ELISA Test Kit
Catalog No. LSY-10051
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Doxycycline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Doxycycline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Doxycycline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Doxycycline in it. This value is compared to the standard curve and the Doxycycline concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue, Honey, Egg 4ppb
Cross-reaction rate:
Doxycycline 100%
Tetracycline 150%
Minocycline 92%
Pyrithione 76%
Chlortetracycline 25%
Demethylchromycin 28%
Oxytetracyline 18%
Recovery rate:
Tissue, Honey, Egg 70%～120%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 15ml | white cap |
9 | 20× sample extract A | 15ml | yellow cap |
10 | 2× sample extract B | 50ml*2 | transparent cap |
11 | 20× sample diluent | 10ml | black cap |
4. Materials required but not provided
1) Equipments: microplate reader, homogenizer, oscillator, centrifuge, balance (a sensibility reciprocal of 0.01 g), graduated pipette, incubator.
2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and multi-channel 30~300 µL;
3) Reagents: NaOH.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
