Nitroimidazole ELISA Test Kit for fish shrimp safety
- Sep 01, 2026 Anon
Nitroimidazole ELISA Test Kit
Catalog No. LSY-10047
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Nitroimidazole in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Nitroimidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Nitroimidazole antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Nitroimidazole in it. This value is compared to the standard curve and the Nitroimidazole concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1ppb
Incubation Temperature: 4℃～25℃～25℃
Incubation Time: 60min～20min～15min
Detection limit
Tissue: 0.3ppb
Egg: 0.5ppb
Cross-reaction rate
Metronidazole 100%
Hydroxy metronidazole 47%
Tinidazole 2%
Dimenidazole 800%
Hydroxymethyl Metronidazole 53%
Ornidazole 5%
Recovery rate: 60%～120%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | High concentration standard | 1ml | 100ppb |
4 | Enzyme conjugate | 12ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× concentrated washing buffer | 15ml | white cap |
10 | Sample redissolving solution | 50ml*2 | yellow cap |
11 | 20× sample extracting buffer | 15ml | transparent cap |
4. Materials required but not provided
1） Equipments: microplate reader (450nm, 630nm), printer, nitrogen-drying device, vortex, shaker, centrifuge (3000g and above), measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator (4℃，25℃), water bath, timer;
2） Micropipettors: single-channel 20-200 µL, 100-1000 µL, and eight-channel 30～300 µl;
3） Reagents (AR): Ethyl acetate, n-hexane, anhydrous sodium sulfate
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
