Metronidazole ELISA Test Kit for honey safety
- Sep 01, 2026 Anon
Metronidazole ELISA Test Kit (Honey)
Catalog No. LSY-10048
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Metronidazole in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Metronidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Metronidazole antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Metronidazole in it. This value is compared to the standard curve and the Metronidazole concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05ppb
Detection limit
Honey: about 0.1ppb
Note: ppb= ng/ml or ng/g
Cross-reaction rate
Metronidazole 100%
Ornidazole 83%
Secnidazole 110%
Metronidazole-OH <1%
Tinidazole <1%
Dimetridazole <1%
Recovery rate: 90±30%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1mL each): 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb
3） Enzyme conjugate (12 mL) 1 bottle
4） Antibody working solution (7mL) 1 bottle
5） Substrate A solution (7 mL) 1 bottle
6） Substrate B solution (7 mL) 1 bottle
7） Stop solution (7 mL) 1 bottle
8） 20× concentrated washing buffer (30 mL) 1 bottle
9） Redissolving solution (50 mL) 1 bottle
4. Materials required but not provided
1） Equipments: microplate reader (450nm, 630nm), printer, nitrogen-drying device, vortex, shaker, centrifuge (3000g and above), measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator (4℃，25℃), water bath, timer;
2） Micropipettors: single-channel 20-200 µL, 100-1000 µL, and eight-channel 30～300 µl;
3） Reagents (AR): Na2HPO4▪12H2O，NaH2PO4·2H2O，Ethyl acetate, Hexane, deionized water.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
