Erythromycin ELISA test kit for fish shrimp crab safety
- Sep 01, 2026 Anon
Erythromycin ELISA test kit
Catalog No. LSY-10050
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Erythromycin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Erythromycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Erythromycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Erythromycin in it. This value is compared to the standard curve and the Erythromycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit: Tissue, egg, milk 0.1ppb
Cross-reaction rate:
Erythromycin 100%
Recovery rate:
Tissue,egg, milk 95% ±30%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | High concentration standard | 1ml | 100ppb |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 2× concentrated redissolving solution | 50ml | transparent cap |
10 | 20× concentrated washing buffer | 40ml | white cap |
4. Materials required but not provided
1) Equipments: microplate reader, rotary evaporator/nitrogen-drying device, homogenizer, oscillator, centrifuge, balance (a sensibility reciprocal of 0.01 g), measuring pipets, incubator.
2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and multi-channel 30~300 µL;
3) Reagents: Ethyl acetate, acetonitrile, n-hexane, sodium hydroxide(NaOH).
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
