Tilmicosin ELISA test kit for meat
- Sep 01, 2026 Anon
Tilmicosin ELISA test kit
Catalog No. LSY-10053
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tilmicosin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Tilmicosin in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Tilmicosin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tilmicosin in it. This value is compared to the standard curve and the Tilmicosin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5ppb
Incubation Temperature: 25℃
Incubation Time: 20min～10min
Detection limit:
Pork, Chicken 2ppb
Recovery rate
Chicken, Pork 90±30%
Cross-reaction rate:
Tilmicosin 100%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.5ppb |
1.5ppb | 4.5ppb | ||
13.5ppb | 40.5ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 15ml | white cap |
9 | 5× Sample extract solution | 50ml×2 | transparent cap |
4. Materials required but not provided
1) Equipments: microplate reader, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g), incubator, printer.
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 μl.
3) Reagents: HCl, NaOH.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co.,Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
