Colistin ELISA Test Kit for feed
- Sep 01, 2026 Anon
Colistin ELISA Test Kit
Catalog No. LSY-10056B
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Colistin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Colistin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Colistin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Colistin in it. This value is compared to the standard curve and the Colistin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5ppb
Incubator temperature: 25℃
Incubator time: 30min～15-20min
Detection limit
Tissue, egg about 20ppb
Raw milk about 30ppb
Finished milk about 20ppb
Feed about 50ppb
Note: ppb= ng/ml or ng/g
Cross-reaction rate
Colistin 100%
Recovery rate
90±30%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 5× standard solution (1mL each): 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb
3） 11X Concentrated Enzyme conjugate (0.7 mL)
4） Enzyme conjugate dilution (7 mL)
5） Substrate A (7 mL)
6） Substrate B (7 mL)
7） Stop solution (7 mL)
8） 20× concentrated washing buffer (30 mL)
9） Redissolving solution (50 mL)
10） Tissue sample extracting buffer-2 (1 mL)
4. Materials required but not provided
1） Equipment: microplate reader (450nm, 630nm), printer, homogenizer, nitrogen-drying device, vortex, shaker, centrifuge (4000g and above), measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator (25℃), timer;
2） Micropipettors: single-channel 20-200 µL, 100-1000 µL, and eight-channel 30～300 µl;
3） Reagents (AR): Deionized water, HCl, H2SO4, Methanol, NaCl.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
