Azithromycin ELISA Test Kit for meat
- Sep 01, 2026 Anon
Azithromycin ELISA Test Kit
Catalog No. LSY-10057
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Azithromycin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Azithromycin in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Azithromycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Azithromycin in it. This value is compared to the standard curve and the Azithromycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05ppb
Incubation Temperature: 25℃
Incubation Time: 30min～15min
Detection limit:
Tissue, egg, milk 0.5ppb
Recovery rate
Tissue, egg, milk 70%~120%
Cross-reaction rate:
Azithromycin 100%
Erythromycin < 1%
Tylosin 30%
Tilmicosin 23%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.05ppb |
0.15ppb | 0.45ppb | ||
1.35ppb | 4.05ppb | ||
3 | Spiking standard solution | 1ml | 100ppb |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× concentrated washing buffer | 40ml | white cap |
4. Materials required but not provided
1) Equipments: microplate reader, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g), incubator.
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 μl.
3) Reagents: Methanol
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co.,Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
