Spectinomycin ELISA Test Kit for seafood
- Sep 01, 2026 Anon
Spectinomycin ELISA Test Kit
Catalog No. LSY-10059
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Spectinmycin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Spectinmycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Spectinomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Spectinomycin in it. This value is compared to the standard curve and the Spectinomycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5ppb
Incubation Temperature: 25℃
Incubation Time: 30min～15min
Detection limit
Tissue 10ppb
Cross-reaction rate
Spectinomycin: 100%
Recovery rate
Tissue 95%±25%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.5ppb |
1.5ppb | 4.5ppb | ||
13.5ppb | 40.5ppb | ||
3 | High concentration standard | 1ml | 1 ppm |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× concentrated washing buffer | 15ml | white cap |
10 | 20× Extracting buffer A | 15ml | yellow cap |
11 | 2× Extracting buffer B | 50ml*2 | transparent cap |
12 | 10× Sample diluent | 10ml | black cap |
4. Materials required but not provided
1） Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator;
2） Micropipettors: single-channel 20-200µL, 100-1000µL, and multi-channel 30～300µl;
3） Reagents: Deionized water, NaOH
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com
