Apramycin Residue ELISA test Kit for seafood
- Sep 01, 2026 Anon
Apramycin Residue ELISA test Kit
Catalog No. LSY-10072
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Apramycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Apramycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Apramycin in it. This value is compared to the standard curve and the Apramycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit
Tissue, honey, egg, milk 1.5ppb
Milk powder 5ppb
Cross-reaction rate
Apramycin 100%
Kanamycin <1%
Gentamicin <1%
Spectinomycin <1%
Streptomycin <1%
Neomycin <1%
Recovery rate
Tissue, honey, egg, milk, milk powder 90% ±30%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.05ppb |
0.15ppb | 0.45ppb | ||
1.35ppb | 4.05ppb | ||
3 | High-concentration standard | 1ml | 1ppm |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 2× Sample dilution | 50ml | transparent cap |
10 | Concentrated extracting buffer | 50ml | yellow cap |
11 | 20× concentrated washing buffer | 15ml | white cap |
4. Materials required but not provided
1) Equipment: microplate reader, printer, homogenizer, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01g), incubator.
2) Micropipette: single-channel 20-200µL, 100-1000µL.
3) Reagents: deionized water.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
