Tiamulin ELISA Test Kit for feed
- Sep 01, 2026 Anon
Tiamulin ELISA Test Kit
Catalog No. LSY-10076
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Tiamulin in samples. The coupling antigens are pre-coated on the micro-well stripes. The Tiamulin in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti- Tiamulin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Tiamulin concentration in the sample. This value is compared to the standard curve and the Tiamulin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.03ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Tissue, egg 1ppb
Feed 2ppb
Recovery rate:
Tissue, egg, feed 90 ±30%
Cross-reaction rate:
Tiamulin 100%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 5× standard solution (1mL each) | 0ppb | 0.03ppb |
0.09ppb | 0.27ppb | ||
0.81ppb | |||
3 | High concentration standard | 1ml | 100ppb |
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A solution | 7ml | white cap |
6 | Substrate B solution | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 2×concentrated redissolving solution | 50ml | transparent cap |
4. Materials required but not provided
1) Equipment: microplate reader, homogenizer, printer, nitrogen-drying device, oscillator, centrifuge, measuring pipets, balance( a reciprocal sensibility of 0.01 g), incubator.
2) Micropipette: single-channel 20-200µL and 100-1000 µL, and multi-channel 30～300 μl;
3) Reagents: Anhydrous methanol, Anhydrous ethanol
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Tip: If there is any air leakage in the vacuum packaging bag of the enzyme-linked immunosorbent assay (ELISA) plate, the ELISA plate is still valid and does not affect the experimental results. Please feel free to use it.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
