Clenbuterol ELISA Test Kit for feed safety
- Sep 01, 2026 Anon
Clenbuterol ELISA Test Kit
Catalog No. LSY-10012
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Clenbuterol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Clenbuterol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Clenbuterol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Clenbuterol in the sample. This value is compared to the standard curve and the Clenbuterol residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit
Urine 0.1 ppb
Tissue (method one) 0.4 ppb
Tissue (method two) 0.1 ppb
Feed 1 ppb
Recovery rate
Urine 95±22%
Tissue 75±20%
Feed 80±20%
Cross-reaction rate
Clenbuterol 100%
Terbutalin <1%
Mabuterol <1%
Brombuterol <1%
Salbutamal < 1%
Ractopamine < 1%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Spiking standard solution | 1ml | 100ppb |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 10ml | blue cap |
6 | Substrate A solution | 7ml | white cap |
7 | Substrate B solution | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
4. Materials required but not provided
1) Equipment: microplate reader, printer, homogenizer, nitrogen-drying device, vortex, oscillator, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g)
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 μl;
3) Reagents: Acetonitrile (CH3CN), NaOH, ethyl acetate, methanol, n-Hexane, HCI (approx 36.5%), Na2 SO4(100%), deionized water
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Remarks: If the vacuum package of microtiter plates has leakage, the microtiter plate is normal and effective, do not affect the experimental result. Please feel free to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com www.lsybt.com
