Ractopamine ELISA Test Kit for urine
- Sep 01, 2026 Anon
Ractopamine ELISA Test Kit
Catalog No. LSY-10013
1. Principle
The test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the sample. The conjugated antigens is pre-coated on the micro-well stripes. The Ractopamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Ractopamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ractopamine concentration in the sample. The value is compared to the standard curve and the Ractopamine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Urine 0.1ppb
Tissue 0.2ppb
Cross-reactions
Ractopamine 100%
Dobutamine <1%
Salbutamol <0.1%
Clenbuterol <0.1%
Recovery rate
Urine, tissue 60~120%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.05ppb |
0.15ppb | 0.45ppb | ||
1.35ppb | 4.05ppb | ||
3 | Spiking standard solution | 1ml | 100ppb |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× concentrated washing buffer | 15ml | white cap |
10 | 2× concentrated extracting solution | 50ml*2 | transparent cap |
4. Materials required but not provided
1) Equipment: microplate reader, printer, homogenizer, nitrogen-drying device, oscillator, centrifuge, measuring pipets, balance ( a reciprocal sensibility of 0.01 g)
2) Micropipettors: single-channel 20-200 µL and 100-1000 µL, and multi-channel 30-300 µL;
3) Reagents: NaOH, HCl
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
