β-agonists ELISA Test Kit For Feed
- Sep 02, 2026 Anon
β-agonists ELISA Test Kit
Catalog No. LSY-10036
1. Principle
The GreenSpring®β-agonists test kit is based on the indirect competitive enzyme immunoassay for the detection of β-agonists in the sample. The coupling antigen is pre-coated on the micro-well stripes. The β-agonists in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-β-agonists antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the β-agonists in the sample. This value is compared to the standard curve and the β-agonists residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 25℃
Incubation Time: 30min～15min
Detection limit:
Porcine urine 0.3ppb
Pork (method 1) 0.6ppb
Pork (method 2) 0.2ppb
Feed 1 ppb
Cross-reaction rate:
Clenbuterol 100%
Cimaterol <4%
Brombuterol 60%
Mabuterol 108%
Bambuterol <5%
Clorprenaline <1%
Salbutamol 75%
Terbutaline 25%
Penbutolol 19%
Tulobuterol 23%
Fenoterol <0.1%
Ractopamine <0.1%
Recovery rate:
Porcine urine, pork 90%±30%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 10ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 5× sample extracting solution | 50ml | transparent cap |
4. Materials required but not provided
1) Equipments: microplate reader, printer, homogenizer, vortex, oscillator, centrifuge, Incubator, measuring pipets, balance (a sensibility reciprocal of 0.01 g)
2) Micropipettors: single-channel 20-200µL, 100-1000µL, and multi-channel 30-300µL;
3) Reagents: NaOH, HCI, methanol.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Remarks: If the vacuum package of microtiter plates has leakage, the microtiter plate is normal and effective, do not affect the experimental result. Please feel free to use.
Shenzhen Lvshiyuan Biotechnology co.,ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com www.lsybt.com
