Cimeterol ELISA Test Kit For Chicken Safety
- Sep 03, 2026 Anon
Cimeterol ELISA Test Kit
Catalog No. LSY-10073
1. Principle
The test kit is based on the indirect competitive enzyme immunoassay for the detection of Cimeterol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Cimeterol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cimeterol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Cimeterol in it. This value is compared to the standard curve and the Cimeterol residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Urine 0.3 ppb
Tissue (method one) 0.5 ppb
Tissue (method two) 0.2 ppb
Recovery rate
95%±30%
Cross-reaction rate
Cimeterol 100%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Spiking standard solution | 1ml | 100ppb |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 10ml | blue cap |
6 | Substrate A solution | 7ml | white cap |
7 | Substrate B solution | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20x concentrated washing buffer | 15ml | white cap |
10 | 2× concentrated redissolving solution | 50ml | transparent cap |
11 | 5x Sample extraction solution | 50ml | blue cap |
4. Materials required but not provided
1) Equipment: microplate reader, printer, homogenizer, nitrogen-drying device, vortex, oscillator, centrifuge, measuring pipets, incubator, balance (a sensibility reciprocal of 0.01 g)
2) Micropipette: single-channel 20-200 µL and 100-1000 µL, and multi-channel 30~300 µL;
3) Reagents: Ethyl acetate, Acetonitrile (CH3CN), NaOH, HCI (approx 36.5%)
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Remarks: If the vacuum package of microtiter plates has leakage, the microtiter plate is normal and effective, do not affect the experimental result. Please feel free to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
