Diethylstilbestrol ELISA Test Kit For Feed Safety
- Sep 03, 2026 Anon
Diethylstilbestrol ELISA Test Kit
Catalog No. LSY-10022
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Diethylstilbestrol(DES) in the feed, urine, liver, meat, shrimp and fish. The conjugate antigen is pre-coated on the micro-well stripes. The Diethylstilbestrol in the sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibodies against Diethylstilbestrol. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the content of Diethylstilbestrol in the sample. This value is compared to the standard curve and the content of the corresponding Diethylstilbestrol is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 37℃
Incubation Time: 30min—30min—15min
Detection limit
Tissue (Shrimp, fish) 0.2 ppb
Pork/liver, chicken/liver 2 ppb
Muscle tissue(Method Two)………………………………………………..0.1ppb
Urine 0.6 ppb
Feed 20 ppb
Cross-reactions rate
DES 100%
Dienestrol < 0.1%
Hexestrol 5%
Ethinylestradiol < 0.1%
Estriol < 0.1%
Recovery rate
Urine 70 ±10%
Feed 90 ±10%
Tissue 85 ±10%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6 × standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 5× concentrated redissolving solution (50 mL) transparent cap
4. Materials required but not provided
1) Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, votex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g)
2) Micropipettors: single-channel 20-200 µL ,100-1000 µL, and multi-channel 30～300 μl;
3) Reagents: NaOH, Acetonitrile (CH3CN), Acetone, deionized water, H3PO4 (85%), ethyl acetate, CHCl3
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
