Phenylethanolamine A ELISA Test Kit For Meat Safety
- Sep 03, 2026 Anon
Phenylethanolamine A ELISA Test Kit(Tissue, feed)
Catalog No. LSY-10040
1. Principle
The test kit is based on the competitive enzyme immunoassay for the detection of Phenylethanolamine A in the sample. The conjugated antigen is pre-coated on the micro-well stripes. The Phenylethanolamine A in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti- Phenylethanolamine A antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Phenylethanolamine A concentration in the sample. The value is compared to the standard curve and the Phenylethanolamine A concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Tissue 0.2 ppb
Feed 0.5 ppb
Cross-reactions
Phenylethanolamine A 100%
Ractopamine <1%
Salbutamol <1%
Clenbuterol <1%
Recovery rate
Tissue, feed 85±25%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 2× concentrated redissolving solution | 50ml | transparent cap |
4. Materials required but not provided
1) Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, oscillator, centrifuge, measuring pipets, balance ( a reciprocal sensibility of 0.01 g), incubator.
2) Micropipettors: single-channel 20-200 µL and 100-1000 µL, and multi-channel 30-300 µL;
3) Reagents: Ethyl acetate, N-hexane, Glacial acetic acid
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
