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Phenylethanolamine A ELISA Test Kit Urine

  • Sep 03, 2026   Anon

Phenylethanolamine A ELISA Test Kit(Urine)

Catalog No. LSY-10040

1. Principle

The test kit is based on the competitive enzyme immunoassay for the detection of Phenylethanolamine A in the sample. The conjugated antigen is pre-coated on the micro-well stripes. The Phenylethanolamine A in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti- Phenylethanolamine A antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Phenylethanolamine A concentration in the sample. The value is compared to the standard curve and the Phenylethanolamine A concentration is subsequently obtained.

 

2. Technical specifications

Sensitivity: 0.1 ppb

Incubator temperature:  25℃

Incubator time:  30min15min

Detection limit

Urine 0.1 ppb

Cross-reactions

Phenylethanolamine A 100%

Ractopamine <1%

Salbutamol <1%

Clenbuterol <1%

Recovery rate

Urine 95±25%

 

3. Components

1

Micro-well strips

12 strips with 8 removable

 wells each

2

6× standard solution (1mL each)

0ppb

0.1ppb

0.3ppb

0.9ppb

2.7ppb

8.1ppb

3

Enzyme conjugate

7ml

red cap

4

Antibody working solution

10ml

blue cap

5

Substrate A

7ml

white cap

6

Substrate B

7ml

black cap

7

Stop solution

7ml

yellow cap

8

20× concentrated washing buffer

40ml

white cap

 

4. Materials required but not provided

1) Equipments: microplate reader, printer.

2) Micropipettors: single-channel 20-200 µL and 100-1000 µL, and multi-channel 30-300 µL;


5. Storage and expiry date

Storage:  store at 2-8 ℃, not frozen.

Expiry date: 12 months; date of production is on box.

 

Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.

 

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788/86-18165709090

Fax 86-755-28938800

Email:   info@lsybt.com 

www.lsybt.com