Cyproheptadine ELISA Test Kit For Feed Safety
- Sep 03, 2026 Anon
Cyproheptadine ELISA Test Kit
Catalog No. LSY-10041
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Cyproheptadine in urine, tissue sample. The coupling antigens are pre-coated on the micro-well stripes. The Cyproheptadine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Cyproheptadine antibody, add TMB substrate for coloration. The higher Cyproheptadine in the sample, the less ELISA antibody combined at the solid phase, the lighter of the coloration. The optical density (OD) value of the sample has a negative correlation with the Cyproheptadine in it. This value is compared to the standard curve and the Cyproheptadine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.04 ppb
Incubation Temperature: 25 ℃
Incubation Time: 30min—15min
Detection limit
Urine 0.04 ppb
Tissue 0.08 ppb
Feed 0.4 ppb
Cross-reaction rate
Cyproheptadine 100%
Recovery rate
Urine 95±20%
Tissue 85±25%
Feed 85±25%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.04 ppb, 0.12ppb, 0.36ppb, 1.08ppb and 3.24ppb
3) Enzyme conjugate (7 mL) red cap
4) Antibody working solution (7 ml) blue cap
5) Substrate A solution (7 mL) white cap
6) Substrate B solution (7 mL) black cap
7) Stop solution (7 mL) yellow cap
8) 20× concentrated washing buffer (40 mL) white cap
4. Materials required but not provided
1） Equipments: microplate reader, printer, homogeniser, nitrogen-drying device, vortex, centrifuge, measuring pipets, and balance (a sensibility reciprocal of 0.01 g);
2） Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30-300 μl;
3） Reagents: Acetonitrile, CH2Cl2, glacial acetic acid
5. Storage and expiry date
Storage: store at 2 to 8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Remarks: If the vacuum package of microtiter plates has leakage, the microtiter plate is normal and effective, do not affect the experimental result. Please feel free to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
