Dexamethasone ELISA test Kit For Honey
- Sep 03, 2026 Anon
Dexamethasone ELISA test Kit
Catalog No. LSY-10042
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Dexamethasone in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Dexamethasone in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Dexamethasone antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Dexamethasone in it. This value is compared to the standard curve and the Dexamethasone concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1ppb
Incubation Temperature: 25℃
Incubation Time: 30min ～ 15min
Detection limit:
Tissue 0.1 ppb
Feed, milk, honey, urine 0.2 ppb
Milk powder 0.3 ppb
Cross-reaction rate:
Dexamethasone 100%
Recovery rate
Tissue, feed 90±25%
Milk powder 85±25%
Urine, milk, honey…………………………………………………………. 95±25%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 5× concentrated redissolving solution | 50ml | transparent cap |
4. Materials required but not provided
1) Equipments: microplate reader, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g), Incubator, printer.
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 μl.
3) Reagents: Ethyl acetate, Acetonitrile (CH3CN), N-hexane.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co.,Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
