Melamine ELISA Test Kit for Feed Safety
- Sep 03, 2026 Anon
Melamine ELISA Test Kit for Feed
Catalog No. LSY-10026f
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Melamine in the feed sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Feed 50ppb
Cross-reaction rate:
Melamine 100%
Recovery rate:
Feed 85±25%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 1ppb |
3ppb | 9ppb | ||
27ppb | 81ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 15ml | white cap |
9 | 10x sample dilution | 50ml | blue cap |
10 | 8x sample extracting solution | 15ml | yellow cap |
4. Materials required but not provided
1) Equipment: microplate reader, printer, vortex, centrifuge, measuring pipets, balance( a reciprocal sensibility of 0.01 g).
2) Micropipettors: single-channel 20~200 µL, 100~1000 µL; and multi-channel 30～300μl;
3) Reagent: Anhydrous methanol
5. Storage and expiry date
Storage: stored at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
