Olaquindox ELISA Test Kit For Feed Safety
- Sep 03, 2026 Anon
Olaquindox ELISA Test Kit
Catalog No. LSY-10025
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Olaquindox in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Olaquindox in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Olaquindox antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Olaquindox in it. This value is compared to the standard curve and the Olaquindox concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2 ppb
Incubator temperature: 37℃
Incubator time: 30min~30min～15min
Detection limit:
Pork, chicken 0.2 ppb
Porcine liver 1 ppb
Feed 20 ppb
Cross-reaction rate:
Olaquindox 100%
Carbadox <7%
Recovery rate:
Tissue 95%±25%
Feed 70%±20%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.2ppb |
0.6ppb | 1.8ppb | ||
5.4ppb | 16.2ppb | ||
3 | Enzyme conjugate | 12ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 2× concentrated redissolving solution | 50ml | transparent cap |
4. Materials required but not provided
1) Equipments: microplate reader (450nm/630nm), homogenizer, oscillator, centrifuge, measuring pipets, nitrogen-drying device and balance (a sensibility reciprocal of 0.01 g), Incubator.
2) Micropipettors: single-channel 20 to 200µL and 100 to 1000µL and multi-channel 30～300µl;
3) Reagents: Acetonitrile, N-hexane, Al2O3
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
