MQCA ELISA Test Kit For Meat
- Sep 03, 2026 Anon
MQCA ELISA Test Kit
Catalog No. LSY-10046
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of MQCA in the sample. The coupling antigens are pre-coated on the micro-well stripes. The MQCA in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-MQCA antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the MQCA in it. This value is compared to the standard curve and the MQCA concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Tissue 1 ppb
Cross-reaction rate:
MQCA 100%
Olaquindox <0.1%
Recovery rate:
Tissue 85%±10%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb
3） Concentrated Enzyme conjugate (1 mL) red cap
4） Antibody working solution (8 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） Redissolving solution (50 mL) transparent cap
4. Materials required but not provided
1) Equipments: microplate reader (450 nm / 630 nm), homogenizer, oscillator, centrifuge, measuring pipets, nitrogen-drying device and balance (a sensibility reciprocal of 0.01 g), Incubator.
2) Micropipettors: single-channel 20 to 200 µL and 100 to 1000 µL and multi-channel 30～300 µl;
3) Reagents: Ethyl acetate, n-hexane, HCl, NaCl, CH3CN
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
