Carbadox metabolites(QCA) ELISA Test Kit For Meat Safety
- Sep 03, 2026 Anon
Carbadox metabolites(QCA) ELISA Test Kit
Catalog No. LSY-10066
1. Principle
This test kit is based on the indirect competitive one-step enzyme immunoassay for the detection of Carbadox metabolites(QCA) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Carbadox metabolites(QCA) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Carbadox metabolites(QCA) antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Carbadox metabolites(QCA) in it. This value is compared to the standard curve and the Carbadox metabolites(QCA) concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Precision
Coefficient of variation within and between plates..............................<10%
Detection limit:
Tissue 0.5 ppb
Cross-reaction rate:
Carbadox metabolites(QCA) 100%
Olaquindox metabolite (MQCA) 120%
Olaquindox (OLA) <1%
Carbadox(CAR) <1%
Sulfaquinoxaline (SQX) <1%
Recovery rate:
Tissue 70%-120%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each)
| 0ppb | 0.2ppb |
0.6ppb | 1.8ppb | ||
5.4ppb | |||
3 | High concentration standard | 100ppb | |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A solution | 7ml | white cap |
7 | Substrate B solution | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× concentrated washing buffer | 15ml | white cap |
10 | Redissolving solution | 50ml | transparent cap |
11 | Extracting solution | 50ml | yellow cap |
4. Materials required but not provided
1) Equipments: microplate reader (450nm/630nm), homogenizer, oscillator, centrifuge, measuring pipets, nitrogen-drying device and balance (a sensibility reciprocal of 0.01 g), Incubator, printer
2) Micropipettors: single-channel 20~ 200 µL and 100~1000 µL and multi-channel 30～300 µl;
3) Reagents: Ethyl acetate, n-hexane, H2SO4, deionized water.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
