Ivermectin ELISA Test Kit For Milk Safety
- Sep 03, 2026 Anon
Ivermectin ELISA Test Kit
Catalog No. LSY-10070
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Ivermectin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Ivermectin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Ivermectin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ivermectin in it. This value is compared to the standard curve and the Ivermectin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5ppb
Incubation time: 45min
Detection limit
Tissue 1.5ppb
Liquid milk 5ppb
Note: ppb= ng/ml or ng/g
Cross-reaction rate
Ivermectin 100%
Recovery rate
85±10%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1mL each): 0ppb,0.5ppb,1.5ppb, 4.5ppb,13.5ppb,40.5ppb
3） Enzyme conjugate (7 mL)
4） Antibody working solution (7 mL)
5） Substrate A (7 mL)
6） Substrate B (7 mL)
7） Stop solution (7 mL)
8） 20× concentrated washing buffer (40 mL)
9） 2x concentrated redissolving solution (50 mL)
4. Materials required but not provided
1） Equipments: microplate reader (450nm, 630nm), printer, homogenizer, nitrogen-drying device, vortex, shaker, grinder, centrifuge (4000r/min and above), measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator (25℃), timer;
2） Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 250 µl;
3） Reagents (AR): acetonitrile, deionized water, NaCl, Methanol.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
