Nicarbazine ELISA Test Kit For Feed Safety
- Sep 03, 2026 Anon
Nicarbazine ELISA Test Kit
Catalog No. LSY-10063
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Nicarbazine. The coupling antigen is pre-coated on the micro-well stripes. The Nicarbazine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Nicarbazine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Nicarbazine in the sample. This value is compared to the standard curve and the Nicarbazine residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Tissue……………………………...….10ppb
Feed……………………………...…....20ppb
Cross-reaction rate:
Nicarbazine ..........................................100%
Recovery rate:
Tissue, Feed………………………70%-120%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 15ml | white cap |
9 | Sample dilution | 50ml*2 | transparent cap |
4. Materials required but not provided
1) Equipment: ELISA Reader (450 nm/630nm), homogenizer, shaker, centrifuge, balance: 0.01g quantity sensitive, incubator, graduated pipettes, printer
2) Micropipettes: single-channel 20ml ~ 200ml, 100ml ~ 1000ml, multi-channel 30～300 μl
3) Reagents: Acetonitrile.
5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co.,Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
