Bird Flu H5 subtype antibody ELISA test kit for bird
- Sep 09, 2026 Anon
Bird Flu H5 subtype antibody ELISA test kit
(Work for Chicken, duck, goose, etc.)
Catalog No. LSY-30011
1. Brief
This kit is used to detect Bird Flu H5 subtype antibody in serum of poultry, wild birds etc., to assess antibody condition by Bird Flu H5 vaccine poultry and assist diagnosis of serological infected poultry.
This kit use blocking ELISA method, H5 antigen is pre-coated on enzyme micro-well strips, indirect detection of anti-H5 antibodies in serum by anti-monoclonal antibodies compete with antibodies in serum for binding sites in solid-phase vectors. When testing, add diluted serum sample, after incubation, if there is H5 virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-H5 virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
2. Reagents
Code | Item | Spec. | Code | Item | Spec. |
1 | H5 Ag Coated plates 96 wells | 1/2 plate | 6 | Stop solution | 15 ml |
2 | Enzyme Conjugate | 11/22 ml | 7 | Negative control | 2 ml |
3 | 10XConcentrated Washing buffer | 100 ml | 8 | Positive control | 1 ml |
4 | Substrate | 11/22 ml | 9 | Adhesive Foil | 2/4 pieces |
5 | Sample dilution | 100 ml | 10 | Instruction | 1 piece |
3. Materials required but not provided
1) Micropipette: 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells with 450/630nm wavelength.
5) Distilled water or deionized water.
6) Bottle washer or Microplate Washer
4. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.
5. Results
Read the OD value with microplate-reader at 450nm (630nm as reference).
For the test to be valid:
Average OD value of Negative control (N) >0.4;
meanwhile OD value of Positive control (P)/ Average OD value of Negative control (N)<0.4.
Calculation method:
OD value of samples/ Average OD value of Negative control = S/N value
Result judge:
S/N ≥ 0.55, Negative;
S/N < 0.55, Positive.
Specifications: 96 or 96*2 wells/kit.
Expiry date: 12 months.
Storage: Storing at 2-8℃, in the dark.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
