Bird Flu subtype H9 antibody ELISA test kit for poultry
- Sep 09, 2026 Anon
Bird Flu subtype H9 antibody ELISA test kit
Catalog No. LSY-30018
1. Usage
This kit is used to detect Bird Flu subtype H9 antibody in chicken serum, to assess antibody condition by Bird Flu subtype H9 vaccine in chicken farm and assist diagnosis of serological infected chicken.
2. Principle
The Bird Flu subtype H9 antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).
3. Reagents
1 | H9 antigen coated microplate | 1/2 plate of 96 wells | 7 | Negative control | 0.8/1.6ml |
2 | Enzyme conjugate | 11/22 ml | 8 | Positive control | 0.8/1.6ml |
3 | 10×concentrated washing buffer | 100ml | 9 | Serum dilution plate | 1/2 piece |
4 | Substrate solution | 11/22 ml | 10 | Adhesive film | 2/4 pieces |
5 | Sample dilution | 50/100ml | 11 | Instruction | 1 piece |
6 | Stopping solution | 11ml | 12 |
4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL
2) 37 ℃ Incubator
3) 96 wells microplate reader
4) Distilled water or deionied water
5) Bottle or microplate washing machine
5. Results
Read the OD value with microplate-reader at 450nm (630nm as reference).
For the test to be valid:
OD value of Negative control (N) < 0.15, meanwhile OD value of Positive control (P) 〉0.4.
Calculation method:
OD value of samples/ Average OD value of Positive control = S/P value
Result judge:
S/P ≥ 0.25, Positive.
S/P < 0.25, Negative;
Specifications: 96*2 wells/kit.
Expiry date: 12 months.
Storage: Storing at 2-8℃, in the dark.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
